LOCUS       pLV-[hsa-miR-886]       9175 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7096..7956
                     /label=Amp(R)
     promoter        8157..8697
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5701..5935
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6510..6965)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6007..6137
                     /label=SV40 pA
     misc_feature    6138..6351
                     /label=SV40 ori
     misc_feature    5022..5618
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3640
                     /label=intron
     CDS             3687..5012
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3430
                     /label=hsa-mir-886 precursor
BASE COUNT     2161 a      2389 c      2482 g      2143 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gcgagaccgc 
     3121 atgacgcagg cctctcgggg gcggggagca catgcagccc gtccctctcc acatcgtcac 
     3181 tcttctatgg tttagaagtt tcagtcgcac actcctaccc gggtcggagt tagctcaagc 
     3241 ggttacctcc tcatgccgga ctttctatct gtccatctct gtgctggggt tcgagacccg 
     3301 cgggtgctta ctgacccttt tatgcaataa attcggtata atctgtcact ctgaaggctt 
     3361 tgttattttt tatccctttt aactttgcta aattaatagg ctgataacat aagttggtgt 
     3421 cactttgaag gctagctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 
     3481 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 
     3541 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 
     3601 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa aactacccct 
     3661 ctagagtcga gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca 
     3721 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 
     3781 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 
     3841 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 
     3901 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 
     3961 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 
     4021 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca 
     4081 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 
     4141 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 
     4201 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 
     4261 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 
     4321 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 
     4381 acgagctgta caaggaccca ccggtcgcca ccatgaccga gtacaagccc acggtgcgcc 
     4441 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 
     4501 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 
     4561 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
     4621 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
     4681 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
     4741 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
     4801 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
     4861 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
     4921 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga 
     4981 cctggtgcat gacccgcaag cccggtgcct gagcggccgc gtcgacaatc aacctctgga 
     5041 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 
     5101 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 
     5161 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 
     5221 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 
     5281 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 
     5341 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     5401 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 
     5461 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 
     5521 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 
     5581 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 
     5641 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 
     5701 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 
     5761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
     5821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
     5881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 
     5941 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 
     6001 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     6061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     6121 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 
     6181 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     6241 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     6301 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 
     6361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 
     6421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 
     6481 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 
     6541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     6601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     6661 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     6721 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     6781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     6841 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     6901 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 
     6961 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     7021 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     7081 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 
     7141 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 
     7201 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
     7261 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 
     7321 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 
     7381 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 
     7441 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 
     7501 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
     7561 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 
     7621 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 
     7681 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 
     7741 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 
     7801 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
     7861 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 
     7921 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 
     7981 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     8041 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     8101 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     8161 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     8221 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 
     8281 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     8341 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     8401 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     8461 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     8521 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     8581 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     8641 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     8701 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     8761 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     8821 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 
     8881 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 
     8941 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 
     9001 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 
     9061 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 
     9121 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgc 
//
